Drosophila life cycle
收藏NIAID Data Ecosystem2026-03-07 收录
下载链接：
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE1761
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资源简介：
RNA from six developmental stages during the Drosophila life cycle (0-2hr embryos, 3-16hr embryos, larvae, pupae, male and female adults) was isolated, reverse transcribed in the presence of oligodT and random hexamers and the labeled cDNA was hybridized to these arrays.Each sample was hybridized four times, twice with Cy5 labeling and twice with Cy3 labeling. Keywords: other
本研究提取了果蝇（Drosophila）生命周期中6个发育阶段的RNA，分别为0-2小时胚胎、3-16小时胚胎、幼虫、蛹、雄性成虫与雌性成虫。将提取的RNA在寡聚脱氧胸苷引物（oligodT）与随机六聚体引物（random hexamers）存在的条件下完成逆转录，获得标记后的互补DNA（cDNA），随后将该标记cDNA与此类基因芯片阵列进行杂交。每个样本均重复四次杂交实验，其中两次采用菁染料Cy5进行标记，另外两次采用菁染料Cy3进行标记。关键词：其他
创建时间：
2013-06-12
