Analysis of RPE65 loss of function in mouse retina

To characterize gene response in RPE65-/- mouse model of Leber’s congenital amaurosis during progression of the disease, we analyzed differential gene expression in retinae early in the development of the disease, namely before and at the onset of photoreceptor cell death in knock-out mice of 2, 4 and 6 months of age. Keywords: knock out response over time We compared gene expression in wild type and RPE65-/- retinae at 2, 4 and 6 months of age. Three biological replicates were performed for each of the six conditions analyzed; this series hence contains 18 samples.

为解析莱伯先天性黑蒙（Leber’s congenital amaurosis）RPE65基因敲除小鼠模型在疾病进程中的基因应答特征，本研究针对疾病早期（即2、4、6月龄敲除小鼠发生光感受器细胞死亡（photoreceptor cell death）之前及发病初期）的视网膜组织进行差异基因表达（differential gene expression）分析。
关键词：基因敲除应答 随时间变化
本研究比较了2、4、6月龄野生型（wild type）与RPE65-/-小鼠的视网膜基因表达水平。本次分析包含6种实验条件，每种条件均设置3次生物学重复（biological replicates），因此本数据集共包含18个样本。