Human umbilical cord­derived mesenchymal stem cells (UC­MSCs) induce a global transcriptional reprogramming in cisplatin­damaged human renal proximal tubular epithelial cells (RPTECs).

To gain insights into the genes involved in the protective effect of human umbilical cord­derived mesenchymal stem cells (UC­MSCs) on cisplatin­injured human renal proximal tubular epithelial cells (RPTECs), we performed a genome­wide differential gene expression analysis by Ion AmpliSeqTM Transcriptome sequencing that targets more than 20,000 human genes. Our data indicated that genes involved in the pathways related to mitochondria and energy homeostasis were strongly upregulated by stem cell treatment in respect to cells treated with cisplatin alone, possibly representing a de novo regenerative response induced by UC­MSCs. Overall design: Gene expression profiles of three RPTECs samples (Control, Cispl, and Cispl+UC­MSCs) were generated by Ion AmpliSeq Transcriptome sequencing, in triplicate, using Ion Proton Sequencer.

为了阐明人脐带间充质干细胞（human umbilical cord-derived mesenchymal stem cells, UC-MSCs）对顺铂（cisplatin）损伤的人肾近端小管上皮细胞（renal proximal tubular epithelial cells, RPTECs）的保护作用所涉及的基因，我们采用靶向超过20000个人类基因的Ion AmpliSeq™ 转录组测序技术，开展了全基因组差异基因表达分析。我们的数据显示，相较于仅接受顺铂处理的细胞组，干细胞共处理组中与线粒体及能量稳态相关通路的基因显著上调，这可能代表了人脐带间充质干细胞诱导的全新再生应答反应。整体实验设计：采用Ion Proton测序仪，通过Ion AmpliSeq转录组测序对3组人肾近端小管上皮细胞样本（对照组、顺铂组、顺铂+UC-MSCs组）进行三次生物学重复的基因表达谱检测。