Enhancer Profiling Reveals Regulators of Skeletal Muscle Identity and Reprogramming [RNA-seq]. Enhancer Profiling Reveals Regulators of Skeletal Muscle Identity and Reprogramming [RNA-seq]

Chromatin immunoprecipitation sequencing of H3K4me2, H3K27ac as well as, ATACseq and RNA-seq reveals regulatory landscapes across different muscle groups, as well as in response to chronic exercise or muscle PGC1a overexpression. This work defines the unique enhancer repetoire of skeletal muscle in vivo and reveals that highly divergent exercise-induced or PGC1a-driven epigenomic programs direct partially convergent transcriptional networks. Overall design: Genome-wide maps of chromatin state in skeletal muscle in exercise, sedentary and transgenic PGC1a conditions

针对组蛋白H3赖氨酸4二甲基化（H3K4me2）、组蛋白H3赖氨酸27乙酰化（H3K27ac）的染色质免疫共沉淀测序（Chromatin immunoprecipitation sequencing），结合转座酶可及性测序（ATAC-seq）与RNA测序（RNA-seq），解析了不同骨骼肌群的表观调控图谱，同时揭示了慢性运动或骨骼肌过氧化物酶体增殖物激活受体γ辅助激活因子1α（PGC1a）过表达后的调控特征。本研究明确了体内骨骼肌的独特增强子组库，并证实差异显著的运动诱导或PGC1a驱动的表观基因组程序，可构建部分趋同的转录调控网络。整体实验设计：获取运动、静息及转基因PGC1a过表达状态下的骨骼肌全基因组染色质状态图谱