WGS of Neospora caninum NC-Liverpool tachyoites

WGS was performed for NC-Liverpool tachyzoites grown in vitro in Vero cells. Sequenced reads were quality controlled and groomed, and subsequently aligned to the Vero genome to remove host cell reads, using Bowtie2. This alignment step produced unmapped paired-end reads in forward and reverse FASTQ files, that were then used to assemble the N. caninum apicoplast genome.全基因组测序（Whole Genome Sequencing, WGS）针对在Vero细胞（Vero cells）中体外培养的犬新孢子虫（Neospora caninum）NC-Liverpool株速殖子开展。对获取的测序读段（reads）进行质量控制与数据修整，随后使用Bowtie2软件将读段比对至Vero细胞基因组以移除宿主细胞来源读段。该比对步骤可生成未比对上的双端读段，存储于正向与反向FASTQ格式文件中，后续依托这些读段组装得到犬新孢子虫的顶质体基因组（apicoplast genome）。