DNA hydroxymethylation profiles reveal possible role of highly methylated TLR signaling on Fasciola gigantica excretory/secretory products (FgESPs) modulation of buffalo DCs [hMeDIP-seq]. DNA hydroxymethylation profiles reveal possible role of highly methylated TLR signaling on Fasciola gigantica excretory/secretory products (FgESPs) modulation of buffalo DCs [hMeDIP-seq]

Purpose: To investigate whether FgESP affects the hydroxylmethylation level of DCs Methods: we have profiled the DNA hydroxymethylome by using hydroxymethylated DNA immunoprecipitation sequencing (h)MeDIP-Seq in the current study Results: DNA hydroxymethylation profiles revealed significant changes in the 5-hydroxymethylcytosine (5hmC) levels in 360 genes when DCs were treated with FgESPs. These differentially expressed genes were highly enriched in pathways associated with immune function. Conclusion: For the first time, our study provides a genome-wide mapping of DNA hydroxylmethylation for DCs that interacted with FgESPs. Overall design: In the current study, we have profiled the DNA hydroxymethylome by using high-throughput sequencing. Total DNA was extracted from buffalo PBMC DCs that were treated with FgESP, which was followed byFgESPs, and then performed hydroxymethylated DNA immunoprecipitation sequencing (h)MeDIP-Seq.

研究目的：探究FgESP是否会影响树突状细胞(Dendritic Cells, DCs)的DNA羟甲基化水平。
研究方法：本研究通过羟甲基化DNA免疫沉淀测序(hydroxymethylated DNA immunoprecipitation sequencing, (h)MeDIP-Seq)对DNA羟甲基化组进行解析。
研究结果：当树突状细胞经FgESPs处理后，DNA羟甲基化谱显示360个基因的5-羟甲基胞嘧啶(5-hydroxymethylcytosine, 5hmC)水平发生显著改变。上述差异表达基因显著富集于免疫功能相关通路。
研究结论：本研究首次完成了与FgESPs相互作用的树突状细胞的全基因组DNA羟甲基化图谱绘制。
实验整体设计：本研究通过高通量测序对DNA羟甲基化组进行分析。从经FgESP处理的水牛外周血单个核细胞(Peripheral Blood Mononuclear Cell, PBMC)来源的树突状细胞中提取总DNA，随后进行羟甲基化DNA免疫沉淀测序(hMeDIP-Seq)。