Gene expression in AES-2 clonal isolates of Pseudomonas aeruginosa

Pseudomonas aeruginosa (P. aeruginosa) lung infection is a significant cause of mortality in patients with cystic fibrosis (CF). Most CF patients acquire unique P. aeruginosa strains from the environment; however clonal strains have been identified in CF communities in several countries. Two clonal strains infect 10% to 40% of patients in three CF clinics in mainland eastern Australia. The expression profiles of four planktonically-grown isolates of one Australian clonal strain (AES-2), and four non–clonal CF P. aeruginosa isolates were compared to each other and to the reference strain PAO1 using the Affymetrix P. aeruginosa PAO1 genome array, to gain insight into properties mediating the enhanced infectivity of AES-1. The isolates were subsequently grown as 3-day old biofilms and similarly extracted for RNA and compared as above. Data analysis was carried out using BIOCONDUCTOR software. Keywords: Comparative strain hybridization Four isolates of the AES-2 strain were compared to four non-clonal isolates and to the reference strain PAO1, when grwn both as planktonic and as a biofilm culture.

铜绿假单胞菌（Pseudomonas aeruginosa，P. aeruginosa）肺部感染是囊性纤维化（cystic fibrosis，CF）患者死亡的重要诱因。绝大多数囊性纤维化患者会从环境中获取独特的铜绿假单胞菌菌株，但多个国家的囊性纤维化患者群体中已检出克隆性菌株。在澳大利亚东部大陆的三家囊性纤维化诊疗中心中，两类克隆株分别感染了10%至40%的就诊患者。本研究采用Affymetrix铜绿假单胞菌PAO1基因组芯片，对一株澳大利亚克隆株（AES-2）的4株浮游生长分离株、4株非克隆性囊性纤维化铜绿假单胞菌分离株，以及参考菌株PAO1的基因表达谱进行了对比分析，旨在解析介导AES-1增强感染性的相关特性。随后，将上述分离株以生物膜形式培养3天，同样提取RNA并按前述方式开展表达谱对比。数据分析采用BIOCONDUCTOR软件完成。关键词：菌株比较杂交。当以浮游态和生物膜两种培养方式生长时，本研究共将4株AES-2菌株分离株与4株非克隆性分离株及参考菌株PAO1进行了对比。