Identification of the targets of the oxidative response repressor SisPerR in Saccharolobus islandicus REY15A

In our current study, SisPerR was revealed as a transcriptional repressor in response to oxidative stress in Saccharolobus islandicus REY15A. Deletion of sisperR abolishes the oxidative stress-induced expression of a large number of genes. To identify the target genes directly bound by SisPerR in vivo, chromatin immunoprecipitation-sequencing (ChIP-seq) was performed. Chromatin immunoprecipitation-sequencing (ChIP-seq) for SisPerR was performed using anti-SisPerR antibody in Saccharolobus islandicus REY15A.

本研究发现，SisPerR是冰岛硫化叶菌（Saccharolobus islandicus）REY15A中响应氧化应激的转录阻遏蛋白。sisperR基因的敲除会阻断氧化应激诱导的大量基因的表达。为了鉴定SisPerR在体内直接结合的靶基因，我们开展了染色质免疫共沉淀测序（chromatin immunoprecipitation-sequencing，ChIP-seq）实验。本研究以冰岛硫化叶菌REY15A为实验材料，采用抗SisPerR抗体完成了针对SisPerR的染色质免疫共沉淀测序实验。