Fast 3D Clear: A Fast, Aqueous-Based, Reversible Three-Day Tissue Clearing Method for Adult and Embryonic Mouse Brain and Whole Body

To date a variety of Tissue Clearing methods have emerged as powerful tools to study intact whole organ anatomy and neuronal brain circuits. However, most of the current clearing methods are time consuming and require the use of several organic solvents rendering the tissues incapable of further processing other than light sheet microscopy. Here we developed a new tissue clearing method named Fast Clear. This water-based clearing method requires a small number of organic agents, provides transparent adult mouse brains in short time (less than 3 days) preserving any transgenic or viral/dye fluorophore and is completely reversible maintaining the integrity of the tissue making compatible with any other clearing method and immunohistochemistry

迄今为止，多种组织透明化（Tissue Clearing）技术已成为研究完整器官解剖结构与脑神经环路的有力工具。然而，当前多数透明化方法耗时较长，且需使用多种有机溶剂，使得组织除光片显微镜（light sheet microscopy）成像外，无法开展其他后续处理。本研究开发了一种名为Fast Clear的新型组织透明化技术。该水基透明化方案仅需少量有机溶剂，可在不足3天内实现成年小鼠全脑透明，且能够保留所有转基因、病毒标记或荧光染料标记的荧光基团；该方法完全可逆，可维持组织完整性，能够兼容其他透明化技术与免疫组织化学（immunohistochemistry）实验。