A global wiring diagram of stress response signaling. A global wiring diagram of stress response signaling

We perform RNA-seq on 28 analog sensitive kinase alleles across 12 different conditions. We use this to determine condition specific kinase function on the transcriptional response. Overall design: 392 yeast RNA-seq samples, 29 Strains, in 12 different conditions 28r was sequenced twice. The first time the sequencing quality was low so that library has been excluded. Of the 391 samples, there are a subset of samples that did not include a kinase inhibitor, and are useful only for controlling for strain, and condition effects. These are samples [0-9]*yc, [0-9]*c, and [0-9]*k. These have been excluded from the matrices as after quality control, they were removed from downstream analysis (which includes many matrix operations).

本研究针对12种不同培养条件下的28种类似物敏感性激酶等位基因（analog sensitive kinase alleles）开展RNA测序（RNA-seq）实验，旨在解析不同条件下激酶在转录应答过程中的特异性功能。
实验整体设计：共包含392份酵母RNA测序样本，涉及29株菌株与12种培养条件，其中编号为28r的样本进行了两次测序。首次测序的质量不佳，对应测序文库已被剔除。在剩余的391份样本中，存在一类未添加激酶抑制剂的样本，仅可用于校正菌株与培养条件带来的效应偏差，此类样本的编号格式为[0-9]*yc、[0-9]*c及[0-9]*k。由于在质量控制阶段已将其从包含诸多矩阵运算的下游分析环节中移除，因此这些样本未被纳入后续的矩阵文件中。