ChIP-seq analysis of wild-type and PML KO mESCs

Promyelocytic leukemia (PML) body is a phase-separated nuclear structure composed of various proteins including several chromatin regulators, and physically associates with chromatin, implying its crucial roles for particular genome functions. To investigate roles of PML bodies in transcriptional regulation, we conducted ChIP-seq analysis for histone modifications, including H3K4me3, H3K27ac, H3K27me3, and H3K9ac with wild-type and PML knockout mESCs.早幼粒细胞白血病小体（Promyelocytic leukemia, PML）是一类由多种蛋白质组成的相分离核结构，其中包含多种染色质调控因子，可与染色质发生物理结合，这提示其在特定基因组功能中发挥关键作用。为探究PML小体在转录调控中的功能，我们以野生型与PML敲除的小鼠胚胎干细胞（mouse embryonic stem cells, mESCs）为实验材料，针对H3K4me3、H3K27ac、H3K27me3及H3K9ac等组蛋白修饰开展了染色质免疫共沉淀测序（ChIP-seq）分析。