High-resolution custom array spanning short arm of chromosome 17

The goal of this experiment was to determine the size, genomic extent and gene content of rearrangements involving 17p. To determine size, genomic extent and gene content for each rearrangement, we used a customized tiling-path oligonucleotide microarray spanning the entire 17p chromosomal region. A 4x44k Agilent Technologies (Santa Clara, CA) microarray was designed using the Agilent e-array website. We selected 42,000 probes covering Chr17: 29,000-22,100,000 (hg18), which represents an average distribution of 1 probe per 526 bp.

本实验旨在明确涉及17号染色体短臂（17p）的染色体重排的规模、基因组覆盖范围及基因组成。为确定每一例重排的上述三项特征，我们采用了覆盖整个17p染色体区域的定制化平铺路径寡核苷酸微阵列（tiling-path oligonucleotide microarray）。本研究所用的4x44k微阵列由安捷伦科技（Agilent Technologies，美国加利福尼亚州圣克拉拉）开发，并通过安捷伦e-array网站完成定制设计。我们共选取42000个探针，覆盖17号染色体（Chr17）29000-22100000（人类基因组组装版本hg18）区域，探针平均分布密度为每526个碱基对（base pair）对应1个探针。