Time course of the mRNA expression in wild-type Thermus thermophilus HB8 strain grown on a rich medium (labeling:ENZO). Thermus thermophilus HB8

We observed the expression profile of the total mRNA of wild-type Thermus thermophilus HB8 strain grown in a rich (TT) medium at 70oC. Keywords: time course Overall design: Three wild-type T. thermophilus HB8 strains were pre-cultured at 70oC for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70oC. Cells were collected after 180, 240, 300, 360, 420, 480, 540, 600, 680, and 760 min, and then crude RNA was extracted. The expression of each mRNA at each time point was analyzed on a GeneChip as described under Sample Description Sheet of each sample in this website.

本研究检测了在70℃条件下于丰富培养基（TT培养基）中培养的野生型嗜热栖热菌（Thermus thermophilus）HB8菌株的总mRNA表达谱。
关键词：时间进程（time course）
实验设计概述：将3株野生型嗜热栖热菌HB8接种于3 mL TT培养基（含0.8%蛋白胨、0.4%酵母提取物、0.2%氯化钠、0.4 mM氯化钙及0.4 mM氯化镁，以氢氧化钠调节pH至7.2）中，于70℃预培养16小时。取2 mL上述菌液接种至1 L相同的新鲜培养基中，继续于70℃培养。分别在培养180、240、300、360、420、480、540、600、680及760分钟时收集菌体，随后提取粗总RNA。本研究通过GeneChip（基因芯片）对各时间点下的各mRNA表达水平进行检测，具体操作流程详见本网站各样本对应的样本描述表。