Single nucleus RNAseq of mouse astrocytes after traumatic spinal cord injury

We conducted snRNAseq of mouse astrocytes after traumatic spinal cord injury (SCI). These data reveal transcriptomic similarities and differences among astrocytes in healthy spinal cord and after spinal cord injury. Overall design: Young adult mice, 2 to 4 months of age, were given a full crush SCI. After 28 days, mice with SCI (n=4), and age and sex matched uninjured controls (n=4) were perfused, and tissue samples collected and snRNA extracted and evaluated as described below.

我们针对创伤性脊髓损伤（spinal cord injury, SCI）后的小鼠星形胶质细胞开展了单细胞核RNA测序（snRNAseq）。本数据集揭示了健康脊髓与脊髓损伤后星形胶质细胞之间的转录组学异同。整体实验设计：选取月龄为2至4个月的健康成年小鼠，实施完全挤压型脊髓损伤造模。造模28天后，对4只脊髓损伤小鼠（n=4）以及年龄、性别匹配的4只未损伤对照小鼠（n=4）进行灌流取材，收集组织样本并提取单细胞核RNA，随后按照下述方法完成评估。