Charting the complexity of the marine microbiome through single cell genomics. GORG-tropics

Marine prokaryoplankton represent a complex, global microbiome that plays key roles in biogeochemistry and ocean ecosystem functioning. Yet their genomic composition remains poorly resolved. We employed randomized single cell genomics to recover 12,715 partial genomes from tropical and subtropical, photic ocean. Each sequenced cell was found to be genomically unique, implying limited clonality within prokaryoplankton populations. This genome collection enables accurate functional annotation and genus-level taxonomic assignments, thus offering a model of a reference genome database for complex microbiomes. For more details: https://www.sciencedirect.com/science/article/pii/S0092867419312735?via%3Dihub and https://osf.io/pcwj9/

海洋原核浮游生物（marine prokaryoplankton）是一类复杂的全球性微生物组群，在生物地球化学循环与海洋生态系统功能维持中发挥核心作用。然而目前学界对其基因组组成的解析仍存在较大局限。本研究采用随机单细胞基因组学技术，从热带、亚热带透光层海洋中获得了12,715条部分基因组。经测序分析发现，每一个被测序的细胞在基因组层面均具有独特性，这表明原核浮游生物种群内的克隆性水平较低。该基因组数据集可实现精准的功能注释与属水平分类鉴定，从而为复杂微生物组的参考基因组数据库构建提供了标准范式。更多详细信息可参阅：https://www.sciencedirect.com/science/article/pii/S0092867419312735?via%3Dihub 和 https://osf.io/pcwj9/