Gene Expression Analysis of Ara-C Resistance in AML

Using two independently derived murine BXH2 cell lines, Ara-C resistant derivatives were developed by exposure to increasing concentrations of Ara-C. Microarray analysis comparing the Ara-C resistant cells to their Ara-C sensitive parental cell lines identified potential genes involved in Ara-C resistance. Two highly Ara-C-resistant cell lines, B117H and B140H, were derived from Ara-C-sensitive parental cell lines, B117P and B140P. Variations in gene expression between these Ara-C-resistant and -sensitive sets were studied. Three replicates per cell line.

本研究以两株独立构建的小鼠BXH2细胞系（murine BXH2 cell lines）为初始材料，通过逐步暴露于递增浓度的阿糖胞苷（Ara-C），成功构建了阿糖胞苷耐药的衍生细胞株。通过基因芯片（Microarray）分析对比阿糖胞苷耐药细胞与其对应的阿糖胞苷敏感亲本细胞系的基因表达谱，本研究鉴定出一批参与阿糖胞苷耐药过程的潜在候选基因。两株高阿糖胞苷耐药细胞系B117H与B140H，分别源自亲本阿糖胞苷敏感细胞系B117P与B140P。本研究针对上述阿糖胞苷耐药与敏感细胞组之间的基因表达差异展开了分析，每株细胞系均设置三次生物学重复。