Effect of spineless-cactus mucilage on the in vitro rumen fermentation of cellulose, starch, and protein

SUMMARY The aim of this study was to evaluate the effect of the levels of spineless-cactus mucilage on the in vitro rumen fermentation of cellulose, starch, and protein. A completely randomized experimental design was adopted with a 5 × 3 factorial arrangement consisting of five levels of spineless-cactus mucilage (0, 5, 10, 20, and 40%) and three substrates (carboxymethylcellulose, starch, and trypticase). Treatments were evaluated in a ruminal environment simulated by in vitro incubation at different times of assessment: 0, 3, 6, 12, 24, and 48 h. The incubation procedure was repeated three times, totaling three evaluations per incubation time for each treatment. There was an interaction (P

摘要：本研究旨在评估无刺仙人掌粘液（spineless-cactus mucilage）的添加水平对纤维素、淀粉与蛋白质的体外瘤胃发酵的影响。本实验采用完全随机设计的5×3因子设计方案，设置5个无刺仙人掌粘液添加梯度（0%、5%、10%、20%及40%）与3种发酵底物（羧甲基纤维素（carboxymethylcellulose）、淀粉、胰蛋白胨（trypticase））。各组处理在体外模拟瘤胃环境中进行孵育培养，并于0、3、6、12、24及48小时多个时间点开展发酵评估。孵育实验重复开展3次，即每个处理在每个孵育时间点均完成3次评估。结果存在显著交互作用（P