Analysis of fucoidan-induced global transcriptional changes in bone marrow-derived dendritic cells (BMDCs) using RNA-Seq technology

To investigate the fucoidan-induced global alteration of signalling pathways, we prepared bone marrow-derived dendritic cells (BMDCs) and treated them with fucoidan. We then performed gene expression profiling analysis using RNA-seq data from 6 different cells at one time point. Comparative gene expression profiling analysis of RNA-seq data for fucoidan-treated and vehicle (0.04% DMSO)-treated BMDC cells.

为探究褐藻多糖（fucoidan）诱导的信号通路全局改变，我们制备了骨髓源树突状细胞（BMDCs）并以褐藻多糖对其进行处理。随后，我们针对单个时间点下6份不同的细胞样本的RNA测序（RNA-seq）数据开展基因表达谱分析，并对经褐藻多糖处理与经溶剂对照（0.04%二甲基亚砜，DMSO）处理的BMDCs的RNA测序数据进行了对比基因表达谱分析。