Differentially expressed protein of <i>Burkholderia pseudomallei</i> WT (pre-exposure vs post-exposure (18) to A549 cells) identified using MALDI-TOF/TOF analysis.

aProtein spot corresponding to position on gel (Fig 4 Panel C), bOutput of computer algorithms that predict the presence (1) or absence (-) of signal peptide, cOutput of computer algorithms that predict the subcellular location of protein. Of the up-regulated proteins, the PSORT algorithm predicted that 63.6% were of cytoplasmic, 9.1% of periplasmic, 18.2% of extracellular and 9.1% of multiple localization sites. Amongst the down-regulated proteins, 94.1% were of cytoplasmic in origin and 5.9% were of multiple localization sites. NA: Protein spot not available. Differentially expressed protein of Burkholderia pseudomallei WT (pre-exposure vs post-exposure (18) to A549 cells) identified using MALDI-TOF/TOF analysis.a. 对应凝胶上特定位置的蛋白斑点（图4面板C） b. 计算机算法预测信号肽存在（标记为1）或缺失（标记为-）的输出结果 c. 预测蛋白质亚细胞定位的计算机算法输出结果。在上调蛋白中，PSORT算法预测63.6%为胞质蛋白、9.1%为周质蛋白、18.2%为胞外蛋白、9.1%为多定位位点蛋白。在下调蛋白中，94.1%为胞质起源蛋白，5.9%为多定位位点蛋白。NA：无可用蛋白斑点。 经MALDI-TOF/TOF分析鉴定得到的类鼻疽伯克霍尔德菌（Burkholderia pseudomallei）野生型（WT）差异表达蛋白，其样本对比为暴露于A549细胞前与暴露后(18)的组别。