Role of p53 in mouse iPS cell generation. Mus musculus

We observed a marked increase in efficiency of iPS cell generation when p53 is deleted. Overall design: Gene expression patterns were compared between p53 wt MEF and p53-null MEF cells. Gene expression patterns were also compared between ES cells and MEF.我们观察到，当p53缺失时，诱导多能干细胞（induced pluripotent stem cell, iPS cell）的生成效率显著提升。 总体实验设计：比较了p53野生型小鼠胚胎成纤维细胞（mouse embryonic fibroblast, MEF）与p53缺失型MEF细胞的基因表达模式；同时也比较了胚胎干细胞（embryonic stem cell, ES）与MEF细胞的基因表达模式。