(+)-Catechin and (-)-epigallocatechin gallate: are these promising antioxidant therapies for frozen goat semen?

ABSTRACT The aim of this study was to evaluate the effects of different concentrations of (+)-catechin or (-)-epigallocatechin gallate (EGCG) on goat semen freezability. Poolsof semen were processed (Experiment 1: 0, 15, 25, 50, 75, or 100µM (+)-catechin; Experiment 2: 0, 15, 25, 50, 75, or 100µM EGCG) and frozen. After thawing, the samples were evaluated for kinematics, plasma membrane (PMi) and acrosome integrity, morphology, and oxidative stress, at 0 and 1h. In Experiment 1, at 0h, VSL and VAP were greater (P

摘要 本研究旨在评估不同浓度的(+)-儿茶素（(+)-catechin）或(-)-表没食子儿茶素没食子酸酯（(-)-epigallocatechin gallate, EGCG）对山羊精液冷冻耐受性的影响。将混合精液样本进行处理（实验1：添加0、15、25、50、75或100µM的(+)-儿茶素；实验2：添加0、15、25、50、75或100µM的EGCG）后进行冷冻保存。解冻后，分别在解冻后0小时与1小时，对样本的精子运动学特性、质膜（plasma membrane, PMi）完整性、顶体完整性、形态学特征及氧化应激水平进行检测评估。在实验1的0小时节点，VSL与VAP值显著更高（P