Human cytomegalovirus modulates mTORC1 to redirect protein translation within quiescently infected monocytes

Human cytomegalovirus (HCMV) has profound effect on gene expression during infection of monocytes. We performed RNA-seq and RIBO-seq to analyze the extend to which HCMV reshapes the transciptome and translatome of infected monocytes. Overall design: Comparative gene expression profiling analysis of RNA-seq data for HCMV-infected, GMCSF, or MCSF treated monocytes of total mRNA and polysome-assoacted mRNA

人类巨细胞病毒（Human cytomegalovirus, HCMV）在感染单核细胞期间对基因表达具有显著影响。本研究借助RNA测序（RNA-seq）与核糖体测序（RIBO-seq），解析HCMV重塑受感染单核细胞转录组与翻译组的具体程度。实验设计概述：针对HCMV感染组、粒细胞-巨噬细胞集落刺激因子（GMCSF）处理组或巨噬细胞集落刺激因子（MCSF）处理组的单核细胞，分别提取总mRNA与多聚体结合mRNA，开展RNA-seq数据的比较基因表达谱分析。