Cancer associated mutants of eIF1A impair Rps3/Rps10 binding and enhance scanning of cell cycle genes [Ribo-Seq]

The Ribo-seq analysis demonstrated that eIF1A is predominantly essential for translation of genes with long 5'UTR genes including cell proliferation and cell cycle progression genes. eIF1A depletion causes broad stimulation of initiation in 5'UTRs at near-cognate AUG codons that diminshes the translation initiation fidelity Overall design: Ribo-seq analysis was performed either in contol or eIF1A knockdown MEFs核糖体测序（Ribo-seq）分析显示，真核翻译起始因子1A（eIF1A）主要对携带较长5'非翻译区（5'UTR）的基因的翻译过程至关重要，这类基因涵盖细胞增殖与细胞周期进展相关基因。eIF1A的表达耗竭会广泛激活5'UTR区域内近同源AUG密码子处的翻译起始过程，进而降低翻译起始的保真度。整体实验设计：分别在对照组与eIF1A敲低的小鼠胚胎成纤维细胞（MEFs）中开展了核糖体测序分析。