Mast cells link immune sensing to antigen avoidance behavior [stomach]. Mast cells link immune sensing to antigen avoidance behavior [stomach]

We investigated antigen-specific avoidance behavior in mice, and found that is critically dependent on mast cells. We analyzed gene expression comparing naive versus immunized wild type mice, and versus mast cell-deficient mice at different times following antigen exposure Overall design: Comparative gene expression profiling analysis of RNA-seq data for Cpa3+/+ (wild type) and Cpa3Cre/+ (mast cell-deficient) mice

本研究探究了小鼠的抗原特异性回避行为，发现该行为的发生严格依赖于肥大细胞（mast cells）。我们通过对比未免疫野生型（wild type）小鼠与免疫野生型小鼠，以及抗原暴露后不同时间点的肥大细胞缺陷（mast cell-deficient）小鼠的基因表达水平，开展了相关分析。
实验整体设计：对Cpa3+/+（野生型）与Cpa3Cre/+（肥大细胞缺陷型）小鼠的RNA测序（RNA-seq）数据进行比较基因表达谱分析。