MRTF/SRF link the cytoskeleton and hedgehog pathway in drug-resistant basal cell carcinomas. Mus musculus

We use multi-dimensional genomic analysis using mouse resistant BCCs to identify a non-canonical hedgehog activation pathway driven by the transcription factor, serum response factor (SRF). Overall design: RNA and whole exome sequencing of mouse sensitive and resistant basal cell carcinoma tumors. Additional samples include RNA-seq from ASZ001 resistant BCC cells treated with Vismodegib (Vismo), CCG-1423 (CCG), or vehicle control. Each treatmet contains triplicate samples sequenced using paired-end primers (2X75).本研究借助针对小鼠耐药性基底细胞癌（Basal Cell Carcinoma, BCC）的多维基因组分析，旨在鉴定由转录因子血清反应因子（Serum Response Factor, SRF）介导的非经典Hedgehog激活通路。实验设计概述：对小鼠敏感性及耐药性基底细胞癌肿瘤样本开展RNA测序与全外显子组测序。额外样本涵盖经维莫德吉（Vismodegib, Vismo）、CCG-1423（CCG）或溶剂对照处理的ASZ001耐药性BCC细胞的RNA测序数据。每个处理组均设置3份生物学重复样本，采用双端测序引物（2×75）完成测序。