Image based identification and targeting of cancer stem cells in pancreatic adenocarcinoma (PDAC). Homo sapiens

Purpose: The goals of this study were to identify quantitative gene expression differences between wild type, Musashi1 null, Msuashi2 null and Musashi1/Musashi2 null MIAPaCa2 pancreatic cancer cells Overall design: mRNA profiles of MIA PaCa-2 cancer cells were generated by deep sequencing, in triplicate, using Illumina HiSeq2500.研究目的：本研究旨在鉴定野生型、Musashi1基因敲除型（Musashi1 null）、Musashi2基因敲除型（Musashi2 null）以及Musashi1/Musashi2双基因敲除型的MIAPaCa2胰腺癌细胞之间的定量基因表达差异。实验整体设计：采用Illumina HiSeq2500测序平台对各组MIA PaCa-2胰腺癌细胞进行深度测序，每组设置3次生物学重复。