eIF2α pathway controls memory consolidation via excitatory and somatostatin inhibitory neurons. eIF2α pathway controls memory consolidation via excitatory and somatostatin inhibitory neurons

To study the impact of p-eIF2α reduction in excitatory neurons on translational landscape, we performed both excitatory neuron-specific translational profiling and general proteomic analysis in the dorsal hippocampus of eIF2α cKICaMKIIα and control mice under basal conditions and following learning. Overall design: Ribotag IP and RNA-Seq of mouse hippocampi of eIF2α cKICaMKIIα and control mice

为探究兴奋性神经元内磷酸化eIF2α（p-eIF2α）水平降低对翻译组图谱的影响，本研究在基础状态及学习后两种实验条件下，对eIF2α cKICaMKIIα小鼠及对照小鼠的背侧海马体同步开展兴奋性神经元特异性翻译组谱分析与整体蛋白质组学分析。实验设计概述：对eIF2α cKICaMKIIα小鼠与对照小鼠的海马体进行Ribotag免疫沉淀（Ribotag IP）及RNA测序（RNA-Seq）。