Emergence of bla NDM-1 associated with the aac(6’)-Ib-cr, acrB, cps, and mrkD genes in a clinical isolate of multi-drug resistant Klebsiella pneumoniae from Recife-PE, Brazil

Abstract INTRODUCTION: The emergence of New Delhi metallo-β-lactamase (NDM) is concernig because it reduces the antibiotic therapy options for bacterial infections. METHODS: Resistant and virulent genes from an isolate of Klebsiella pneumoniae derived from a patient with sepsis in a hospital in Recife-PE, Brazil, were investigated using PCR and DNA sequencing. RESULTS: bla NDM-1, aac(6’)-Ib-cr and acrB resistance genes, and cps and mrkD virulence genes were detected. CONCLUSIONS To our knowledge, this is the first report on bla NDM-1 in Recife-PE. This detection alerts researchers to the need to control the spread of bla NDM-1 resistance gene by this bacterium in Brazil.

【引言】新德里金属-β-内酰胺酶（New Delhi metallo-β-lactamase，NDM）的出现引发广泛担忧，因其大幅缩减了细菌性感染的抗生素治疗可选方案。
【研究方法】本研究针对巴西伯南布哥州累西腓市某医院1例脓毒症患者体内分离得到的1株肺炎克雷伯菌（Klebsiella pneumoniae），采用聚合酶链式反应（Polymerase Chain Reaction，PCR）及DNA测序技术，对其耐药基因与毒力基因展开检测分析。
【研究结果】本研究检出bla NDM-1、aac(6’)-Ib-cr及acrB耐药基因，以及cps和mrkD毒力基因。
【研究结论】据我们所知，这是巴西伯南布哥州累西腓市首例bla NDM-1检出报告。此次检出结果警示科研人员，需加强对该细菌在巴西境内扩散bla NDM-1耐药基因的防控工作。