High-speed atomic force microscopy images of DNA origami triangles adsorbing at mica surfaces

Each folder corresponds to a different DNA origami concentration as indicated. Imaging was performed using a JPK Nanowizard ULTRA Speed 3 (Bruker) with USC F0.3-k0.3 cantilevers (NanoWorld). The DNA origami triangles were suspended in 1x TAE (pH 8.5) containing 10 mM CaCl2 and 75 mM NaCl. For the first 10 min, images were recorded with 1 x 1 µm² scan size at a line rate of 200 Hz and a resolution of 200 x 200 pixels resulting in a frame rate of 1 fps. After about 10 min, the resolution was increased to 400 x 400 pixels, lowering the frame rate to 0.5 fps. After about another 15 min, overview images 4 x 4 µm² in size were recorded at a line rate of 20 Hz and a resolution of 2048 x 2048 pixels.每个文件夹对应一个如标注所示的不同DNA折纸（DNA origami）浓度。成像实验使用布鲁克（Bruker）JPK Nanowizard ULTRA Speed 3型原子力显微镜，搭配NanoWorld公司出品的USC F0.3-k0.3型微悬臂。本实验中的DNA折纸三角形结构悬浮于含10 mM氯化钙与75 mM氯化钠的1×TAE缓冲液（pH 8.5）中。实验初始的10分钟内，以1×1 µm²的扫描视野、200 Hz的行扫描速率及200×200像素的分辨率采集图像，此时帧率为1 fps（帧/秒）。约10分钟后，将分辨率提升至400×400像素，帧率随之降至0.5 fps。再经过约15分钟后，采集尺寸为4×4 µm²的全景图像，此时行扫描速率为20 Hz，分辨率为2048×2048像素。