A meta-analysis of plant tissue O2 dynamics

This dataset includes 1,567 recorded tissue O2 levels from 112 plant species extracted from published literature. The data forms the basis of the publication "A meta-analysis of plant tissue O2 dynamics" with the following abstract:

Adequate tissue O2 supply is crucial for plant functioning. We therefore aimed at identifying environmental conditions and plant characteristics affecting plant tissue O2 status. We extracted data and performed meta-analysis on > 1,500 published tissue O2 measurements from 112 species. Tissue O2 status ranged from anoxic conditions in especially roots, to > 53 kPa in submerged, photosynthesizing shoots. Using information-theoretic model selection, we identified ‘submergence’, ‘light’, ‘tissue type’ as well as ‘light × submergence’ interaction as significant drivers of tissue O2 status. Median O2 status was especially low (< 50% of atmospheric equilibrium) in belowground rhizomes, potato tubers and root nodules. Mean shoot and root O2 was ~25% higher in light than in dark when shoots had atmospheric contact. However, light showed a significant interaction with submergence on plant O2, with a submergence-induced 44% increase in light, compared with a 42% decline in dark, relative to plants with atmospheric contact. During submergence, ambient water column O2 and shoot tissue O2 correlated stronger in darkness than in light conditions. Although miniaturised Clark-type O2 electrodes in particular have resulted in enhanced understanding of plant O2 dynamics, the use of non-invasive methods is still lacking behind its widespread use in mammalian tissues.

Methods
We performed a structured and systematic literature search to identify studies reporting O2 measurements in vegetative plant tissues. The search was conducted using the Web of Science BIOSIS database applying Boolean operators, as explained in detail in the Supplementary Materials. The search resulted in a final list of 129 studies published up until September 2022. We extracted each observation of plant tissue O2 status and entered it into a database alongside information of plant species, tissue type, light conditions, submergence status, treatment, O2 measurement technique, tissue excisions status, etc. if provided in the source.

The studies used for this review provide a wide range of ways to measure and report levels of plant tissue O2. Details on data extractions, unit conversions, etc. are available in the related publication. Data were extracted from publication text, tables or graphs using ImageJ. Please also see the accompanied README file for additional information on data structure etc.

本数据集包含从已发表文献中提取的、来自112种植物的1567组组织氧（O2）水平实测记录。该数据集是论文《植物组织氧动态元分析》（A meta-analysis of plant tissue O2 dynamics）的研究基础，论文摘要如下：

充足的组织氧供应是维持植物生理功能的关键。为此，本研究旨在明确影响植物组织氧状态的环境条件与植物固有特性。我们共提取了112个物种的1500余组已发表的组织氧实测数据，并开展元分析（meta-analysis）。植物组织氧状态跨度极大：从特定根系的缺氧环境，到处于淹没状态的光合嫩组织中超过53 kPa的氧水平。通过信息论模型选择方法，我们确认‘淹没’‘光照’‘组织类型’以及‘光照×淹没’交互作用为影响组织氧状态的显著驱动因子。地下根状茎、马铃薯块茎与根瘤中的氧中位水平尤其偏低，仅为大气平衡浓度的50%以下。当嫩组织与大气直接接触时，光照条件下嫩组织与根系的平均氧水平较黑暗条件高出约25%。不过，光照与淹没对植物氧水平存在显著交互作用：以与大气接触的植株为参照，光照条件下淹没会使氧水平提升44%，而黑暗条件下则会下降42%。在淹没状态下，黑暗环境中的水体环境氧浓度与嫩组织氧浓度的相关性较光照环境更强。尽管微型克拉克（Clark）型氧电极的应用极大提升了我们对植物氧动态的认知，但非侵入式测量方法在植物组织中的普及程度仍远落后于其在哺乳动物组织中的应用。

研究方法
我们开展了结构化系统文献检索，以筛选出报道植物营养组织氧含量测量结果的研究。本次检索依托Web of Science BIOSIS数据库完成，采用布尔运算符，具体检索细节详见补充材料。本次检索最终纳入截至2022年9月发表的129项研究。我们提取了每一项植物组织氧状态的观测数据，并将其与植物物种、组织类型、光照条件、淹没状态、实验处理方式、氧测量技术、组织是否离体等相关信息（若原文提供）一并录入数据库。
本综述纳入的各项研究采用了多种多样的植物组织氧水平测量与报告方式。关于数据提取、单位转换等详细信息，可参阅相关研究论文。数据通过ImageJ软件从发表文献的文本、表格或图表中提取。如需了解数据结构等额外信息，亦可参阅随附的README文件。