8-oxoG RNA IP data set 2: Air pollution mixture derived from the reaction of 100 ppb acrolein, 97 ppb methacrolein, 44 ppb alpha-pinene and 100 ppb O3 for 1.5 h

While environmental agents such as air pollution have been shown to be causative to diseases, yet limited knowledge exist on their mechanism of action. Here we used RNA-sequencing in combination with specific immunoprecipitation of 8-oxo-7,8-dihydroguanosine (8-oxoG) to identify transcripts accumulating oxidation after exposure to air pollution (derived from the reaction of acrolein, methacrolein, alpha-pinene and ozone) in bronchial epithelial BEAS-2B cells. Results from this analysis suggest a functional role of the 8-oxoG modified transcripts after air pollution exposure in pathways that are central regulators of cell metabolism, growth, proliferation and maintenance of cellular structure. This study highlights a critical role for a RNA epitranscriptomics modification that can be used to further characterize biological responses to external stressors once considered indistinguishable. 8-oxoG was measured from rRNA depleted RNA in BEAS-2B cells exposed to air pollution mixtures (derived from the reaction of 100 ppb acrolein, 97 ppb methacrolein, 44 ppb alpha-pinene and 100 ppb O3) and clean air for 1.5 hours. Two biological replicates are available from control input, and two biological replicates for IP 8-oxoG RNA.

尽管已有研究表明空气污染等环境因子可引发疾病，但目前学界对其作用机制的认知仍十分匮乏。本研究采用RNA测序（RNA-sequencing）结合8-氧代-7,8-二氢鸟苷（8-oxo-7,8-dihydroguanosine, 8-oxoG）特异性免疫沉淀技术，在支气管上皮BEAS-2B细胞中，筛选出暴露于由丙烯醛、甲基丙烯醛、α-蒎烯与臭氧反应生成的空气污染混合物后，发生氧化积累的转录本。本分析结果显示，空气污染暴露后被8-oxoG修饰的转录本，在调控细胞代谢、生长、增殖及细胞结构维持的核心通路中发挥功能性作用。本研究揭示了一种RNA表观转录组学（epitranscriptomics）修饰的关键作用，借助该修饰可进一步解析曾被视为难以区分的外源应激物所介导的生物学应答。研究人员从暴露于上述空气污染混合物（由100 ppb丙烯醛、97 ppb甲基丙烯醛、44 ppb α-蒎烯与100 ppb臭氧反应生成）以及清洁空气1.5小时的BEAS-2B细胞的核糖体RNA（ribosomal RNA, rRNA）缺失RNA样本中，检测了8-oxoG的水平。本数据集包含对照组输入样本的2个生物学重复，以及8-oxoG RNA免疫沉淀样本的2个生物学重复。