Comparison of class-B GATA overexpression transcriptomes. Arabidopsis thaliana

We have used Agilent whole genome arrays (v4) to compare the gene expression changes between 10-day-old Arabidopsis wild type seedlings and AtGNLox (AT4G26150), AtGNCox (AT5G56860), AtGATA17ox (AT3G16870), BdGATA6ox (BRADI3G08240) as well as the C-terminal mutation constructs AtGNL_LLM/AAAox and AtGNC_LLM/AAAox. Three independent biological samples were prepared for each genotype. Overall design: Three replicate measurements of three biological samples were prepared for each genotype (1-3 or A-C). The experiment was performed in two batches; samples with replicate numbers 1-3 and A-C were prepared and hybridzed in the same respective batch.

本研究采用安捷伦全基因组芯片（Agilent whole genome arrays，v4版），对比分析10日龄拟南芥（Arabidopsis）野生型幼苗与以下转基因株系的基因表达变化：AtGNL过表达株系（AtGNLox，AT4G26150）、AtGNC过表达株系（AtGNCox，AT5G56860）、AtGATA17过表达株系（AtGATA17ox，AT3G16870）、BdGATA6过表达株系（BdGATA6ox，BRADI3G08240），以及C端突变构建体过表达株系AtGNL_LLM/AAAox与AtGNC_LLM/AAAox。所有基因型均制备三份独立生物学重复样本。
实验设计概述：每份基因型设置三份生物学重复样本，并对每份样本开展三次重复检测（编号1-3或A-C）。实验分为两批进行：编号1-3与A-C的样本分别在对应批次中完成制备与杂交。