1f674c6a-01cb-4241-aced-1f6e2aaf0f65 - samples

Bone marrow mononuclear cells from patients diagnosed with B cell precursor acute lymphoblastic leukemia were obtained at three sequential time points: first diagnosis, remission after chemotherapy and relapse. Genomic DNA was isolated and targeted gene panel sequencing was performed using a customized biotinylated RNA oligo pool (SureSelect, Agilent, Santa Clara, California) to hybridize the target regions comprising 362 kbp on a HiSeq2000. Target regions were selected to validate mutations previously identified in these samples using whole exome sequencing.EGA dataset EGAD00001004977

本研究的样本来源于确诊为B细胞前体急性淋巴细胞白血病（B cell precursor acute lymphoblastic leukemia）患者的骨髓单个核细胞，采集于三个连续时间节点：初诊时、化疗后缓解期以及复发阶段。研究人员提取了样本的基因组DNA，并采用定制化生物素化RNA寡核苷酸探针池（SureSelect，安捷伦，加利福尼亚州圣克拉拉市）开展靶向基因面板测序，该探针池可与HiSeq2000测序平台上总长362千碱基对的靶区域进行杂交。本次选取的靶区域用于验证此前通过全外显子测序（whole exome sequencing）在该类样本中鉴定出的突变。本数据集对应EGA数据库编号EGAD00001004977。