The imprinted Zdbf2 gene finely tunes hypothalamic control of feeding and growth in neonates.

To allow identification of differentially expressed genes between WT and Zdbf2-KO hypothalamus in mouse pups at 3 and 10dpp, mutants and control RNA-seq were conducted starting from whole hypothalamus RNA. Overall design: Comparison of RNA-seq results from whole hypothalamus RNA of control and Zdbf2-KO at 3 and 10 dpp.

为鉴定产后3日龄（3dpp）与产后10日龄（10dpp）的小鼠幼崽下丘脑内野生型（Wild Type, WT）与Zdbf2基因敲除（Zdbf2-KO）个体间的差异表达基因，本研究以全下丘脑总RNA为起始材料，开展了敲除组与对照组的RNA测序（RNA-seq）实验。总体实验设计：对比产后3日龄与产后10日龄的野生型及Zdbf2-KO小鼠全下丘脑总RNA的RNA测序结果。