Expression data of β-subunit of Snf1 kinase in yeast Saccharomyces cerevisiae. Saccharomyces cerevisiae

The conserved Snf1/AMPK (AMP-activated protein Kinase) family is one of the central components in nutrient sensing and regulation of carbon metabolism in eukaryotes. It is also involved in several other processes such as stress resistance, invasive growth and ageing. Snf1 kinase is composed of a catalytic α-subunit Snf1, a regulatory γ-subunit Snf4 and one of three possible β-subunits, Sip1, Sip2 or Gal83. We used a systematic approach to study the role of the three β-subunits by analyzing all 7 possible combinations of β-subunit deletions together with the reference strain. Overall design: 7 mutant strains together with 1 reference strain grown under glucose-limited condition with three biological replicates for each strain

保守型Snf1/AMPK（AMP激活蛋白激酶，AMP-activated protein Kinase）家族是真核生物营养感知与碳代谢调控的核心组分之一。该家族同时参与胁迫抗性、侵袭生长与衰老等多项其他生物学过程。Snf1激酶由催化型α亚基Snf1、调控型γ亚基Snf4以及三种可选β亚基（Sip1、Sip2或Gal83）中的任意一种组成。本研究采用系统性研究策略，通过分析β亚基缺失的全部7种可能组合与参考菌株，探究三种β亚基的生物学功能。
实验整体设计：将7株突变菌株与1株参考菌株置于葡萄糖限制培养条件下培养，每株菌株设置3次生物学重复。