Vitamin D sterol effects on coronary ASMC genes. Homo sapiens

Chondro/osteoblastic and cardiovascular-disease associated genes are modulated in human coronary artery smooth muscle cells that calcify in the presence of phosphate and vitamin D sterols. Looking at the effect of media conditions with and without Vitamin D, paracalcitriol (a vitamin D analog), or R568 (a calcimimetic) vs. dose and time on calcification of human coronary smooth muscle cells. Overall design: CASMCs were exposed to differentiation medium [beta glycerophosphate (10 mM), ascorbic acid (50 mg/ml), and dexamethasone (10-9 M) with and without calcitriol or paricalcitol (10-8 M) for 7 days, and total RNA was examined by microarray.

在暴露于磷酸盐与维生素D甾醇并发生钙化的人冠状动脉平滑肌细胞中，软骨细胞/成骨细胞相关及心血管疾病相关基因的表达受到调控。
本研究旨在考察培养基条件（分别添加/不添加维生素D、帕里骨化醇（paracalcitriol，一种维生素D类似物）或R568（钙敏剂，calcimimetic））以及剂量与时间因素对人冠状动脉平滑肌细胞钙化的影响。
总体实验设计：将人冠状动脉平滑肌细胞（coronary artery smooth muscle cells，CASMCs）置于分化培养基（含10 mM β-甘油磷酸钠、50 mg/ml抗坏血酸、10^-9 M地塞米松，分别添加/不添加10^-8 M骨化三醇（calcitriol）或帕里骨化醇（paricalcitol））中培养7天，随后通过微阵列技术检测总RNA的表达水平。