DataSheet_1_No evidence on infectious DNA-based agents in pediatric acute lymphoblastic leukemia using whole metagenome shotgun sequencing.docx

The etiology of pediatric acute lymphatic leukemia (ALL) is still unclear. Whole-metagenome shotgun sequencing of bone marrow samples in patients with treatment-naïve ALL (n=6) was performed for untargeted investigation of bacterial and viral DNA. The control group consisted of healthy children (n=4) and children with non-oncologic diseases (n=2) undergoing bone marrow sampling. Peripheral blood of all participants was investigated at the same time. After bioinformatical elimination of potential contaminants by comparison with the employed controls, no significant amounts of microbial or viral DNA were identified.

儿童急性淋巴细胞白血病（pediatric acute lymphatic leukemia, ALL）的病因迄今尚未明确。本研究对6例初治ALL患者的骨髓样本开展全宏基因组鸟枪法测序（whole-metagenome shotgun sequencing），以非靶向方式检测其中的细菌与病毒DNA。对照组纳入4名健康儿童及2名接受骨髓采样的非肿瘤性疾病患儿。同时对所有受试者的外周血进行了检测。经与所用对照组比对并通过生物信息学方法剔除潜在污染物后，未检出显著量的微生物或病毒DNA。