GSE47237: H3K27Ac.14-16 hr OR embryo.Solexa

Summary: modENCODE_submission_4120 This submission comes from a modENCODE project of Gary Karpen. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We aim to determine the locations of the major histone modifications across the Drosophila melanogaster genome. The modifications under study are involved in basic chromosomal functions such as DNA replication, gene expression, gene silencing, and inheritance. We will perform Chromatin ImmunoPrecipitation (ChIP) using the Illumina NGS sequencing platform. We will initially assay localizations using chromatin from three cell lines and two embryonic stages, and will then extend the analysis of a subset of proteins to four additional animal tissues/stages. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Overall Design: EXPERIMENT TYPE: CHIP-seq. BIOLOGICAL SOURCE: Strain: Oregon-R(official name : Oregon-R-modENCODE genotype : wild type ); Developmental Stage: Embryo 14-16hr OR; Genotype: wild type; EXPERIMENTAL FACTORS: Strain Oregon-R(official name : Oregon-R-modENCODE genotype : wild type ); Antibody H3K27Ac (target is H3K27ac); Developmental Stage Embryo 14-16hr OR

摘要：modENCODE提交项4120
本提交项源自Gary Karpen主导的modENCODE项目。若需查看modENCODE项目的完整列表，请访问http://www.genome.gov/26524648。

项目目标：本研究旨在定位黑腹果蝇（Drosophila melanogaster）基因组中主要组蛋白修饰的分布位点。所研究的修饰参与了DNA复制、基因表达、基因沉默与遗传等核心染色体生物学功能。我们将依托Illumina下一代测序（Next Generation Sequencing, NGS）平台开展染色质免疫沉淀（Chromatin ImmunoPrecipitation, ChIP）实验。首先将使用3种细胞系及2个胚胎阶段的染色质进行位点定位检测，后续还会将部分蛋白的分析拓展至另外4种动物组织/发育阶段。有关数据使用的条款与条件，请参阅http://www.genome.gov/27528022及http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf。

整体实验设计：
实验类型：ChIP-seq
生物样本来源：品系：Oregon-R（官方名称：Oregon-R-modENCODE，基因型：野生型）；发育阶段：胚胎14-16小时（Oregon-R品系）；基因型：野生型
实验因素：品系：Oregon-R（官方名称：Oregon-R-modENCODE，基因型：野生型）；抗体：H3K27Ac（靶标为H3K27乙酰化修饰）；发育阶段：胚胎14-16小时（Oregon-R品系）