Path-seq identifies essential mycolate remodeling program for mycobacterial adaptation in host cells [RNA-seq]

Comparison of transcriptional differences (RNA-seq) between induced and uninduced overexpression of MSMEG_0916 in Mycobacterium smegmatis Overall design: Cultures of Mycobacterium smegmatis with pDTCF-MSMEG_0916 were grown in the presence or absence of anhydrotetracycline (ATc) for 4 hour and harvested for mRNA expression profiling by Illumina sequencing, in triplicate. The sequence reads that passed quality filters were aligned with Bowtie2 and processed using the R package DuffyNGS.

耻垢分枝杆菌（Mycobacterium smegmatis）中MSMEG_0916诱导与未诱导过表达样本间转录差异的RNA测序（RNA-seq）比较。实验设计：携带pDTCF-MSMEG_0916质粒的耻垢分枝杆菌培养液分别在添加与不添加脱水四环素（anhydrotetracycline, ATc）的条件下培养4小时，随后收集样本进行mRNA表达谱分析，采用Illumina测序技术，设置三次生物学重复。通过质量过滤的测序reads将通过Bowtie2进行序列比对，并使用R包DuffyNGS完成后续数据处理。