Bulk RNA-seq of multi-tissue transcriptomes from control and DHEA-treated mice (ovary, uterus, thymus, brain, heart, intestine, kidney, liver and spleen)

To characterize systemic transcriptional changes induced by DHEA treatment, we performed bulk RNA-seq across a panel of tissues collected from control and DHEA-treated mice. Total RNA was extracted from each tissue using a kit-based workflow following mechanical homogenization in lysis buffer. RNA concentration/quality were assessed prior to library construction and high-throughput sequencing. Overall design: Control and DHEA-treated mice were included. For each group, tissues were collected for transcriptomic profiling, including ovary, uterus, thymus, brain, heart, intestine, kidney, liver and spleen. Immediately after dissection, tissues were placed into lysis buffer, homogenized using a tissue homogenizer, and total RNA was purified using a commercial RNA extraction kit. Purified RNA was quantified and quality-checked, then submitted for bulk RNA-seq library preparation and sequencing.

为表征脱氢表雄酮（DHEA）处理诱导的系统性转录组变化，我们对取自对照组及DHEA处理组小鼠的多组织样本开展了批量RNA测序（bulk RNA-seq）。我们采用基于试剂盒的实验流程，先在裂解缓冲液中对每份组织进行机械匀浆，再从中提取总RNA。在文库构建与高通量测序（high-throughput sequencing）之前，我们对RNA的浓度与质量进行了评估。
实验整体设计：本研究纳入对照组与DHEA处理组小鼠。两组均收集组织以开展转录组分析（transcriptomic profiling），所涉组织包括卵巢、子宫、胸腺、脑、心脏、肠道、肾脏、肝脏与脾脏。
组织离体后即刻置于裂解缓冲液中，使用组织匀浆仪进行匀浆，随后通过商业化RNA提取试剂盒纯化总RNA。纯化后的RNA会进行浓度定量与质量检测，随后提交至批量RNA测序文库制备与测序环节。