Richard Allen (2010) CIL:9929, Didinium nasutum, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. CIL. Dataset

A high resolution image of toxicysts in the cytoplasm of cross sectioned non-dividing Didinium. Toxicysts (extrusomes) are multilayered cylinders that are discharged from the cytopharynx region during capture of prey such as Paramecium. Lipid is stored as large non-membrane enclosed dense bodies in the cytosol. TEM taken on 2/18/69 by R. Allen with Philips 300. Neg. 20,500X. The raw film was scanned with an Epson Perfection V750 Pro. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

高分辨率的细胞质中裂解未分裂 Didinium 细胞的毒性囊泡图像。毒性囊泡（外泌体）为多层圆柱状结构，在捕食诸如草履虫等猎物时自胞咽区域排出。脂质以大型非膜包裹的致密体形式储存于细胞质中。图像于1969年2月18日由R. Allen使用Philips 300型透射电子显微镜拍摄，负片放大倍数为20,500倍。原始胶片使用Epson Perfection V750 Pro扫描仪进行扫描。采用标准戊二醛固定法，随后用四氧化锇处理，经酒精脱水并浸泡于环氧树脂中。采用厚度约为75纳米的切片机制备切片。更多详细信息可查阅（http://www5.pbrc.hawaii.edu/allen/）。