Lactobacillus rhamnosus GG strain:GG Genome sequencing

Induced mutagenesis was used to study the pilus biogenesis in L.rhamnosus GG. A combination of two powerful approaches, mutation selection and next generation sequencing, were applied to L.rhamnosus GG for the selection of pilus-deficient mutants from an enriched population.本研究采用诱导诱变技术，探究鼠李糖乳杆菌GG（L.rhamnosus GG）的菌毛生物发生过程。本研究结合突变筛选与下一代测序两种高效研究策略，对鼠李糖乳杆菌GG进行处理，从富集菌群中筛选得到菌毛缺陷突变株。