16S rRNA gene-based primer pair showed high specificity and quantification accuracy in detecting freshwater Brocadiales anammox bacteria

29 samples of various types and from different locations were used to evaluate a primer pair devised to target freshwater Brocadiales anammox bacteria. Illumina amplicon sequencing reads using this primer pair as well as bacterial primer pair were obtained.

本研究选取29份类型各异、采自不同地域的样本，用于评估一套专为靶向淡水*Brocadiales*目厌氧氨氧化（anammox）细菌设计的引物对（primer pair）；同时获取了使用该特异性引物对与通用细菌引物对进行扩增后得到的Illumina扩增子测序读段。