RNA-seq of perihaematomal and anatomically matched contralateral brain tissue after intracerebral hemorrhage

Secondary injury causes death and dependence after spontaneous intracerebral haemorrhage (ICH). Having found that ICH is associated with activation of genes regulated by the transcription factor NRF2, we performed bulk RNA sequencing of perihaematomal and anatomically matched contralateral brain homogenate obtained at autopsy from a cohort of patients who died either within 24h of ICH or between 4-14 days of ICH onset, to investigate the spatiotemporal evolution of this Nrf2 activation.

自发性脑内出血（spontaneous intracerebral haemorrhage, ICH）后，继发性损伤可导致患者死亡或出现功能依赖。本研究团队前期发现ICH与转录因子NRF2（transcription factor NRF2）调控的基因激活存在关联，为此我们对经尸检（autopsy）获取的患者血肿周围（perihaematomal）组织及解剖匹配的对侧脑组织匀浆（anatomically matched contralateral brain homogenate）进行了批量RNA测序（bulk RNA sequencing）；所用患者队列分为两组，分别于ICH发病后24小时内或发病后4至14天死亡，以此探究Nrf2激活的时空动态演化规律。