RNA-seq in AGS-EBV, shRNA

Gastric cancer is one of the most common cancers worldwide. Epstein-barr virus-associated gastric cancer accounts for about 10% of all gastric cancers. EBV itself expresses a variety of proteins and regulates the expression of host genes. In this study, we examined the knockdown of BC200 and eIF4A3 in AGS-EBV, respectively, and analyzed its effect on host mRNA expression. Overall design: Total RNA was prepared using Trizol reagent from nine each independent cultures of AGS-EBV cells.

胃癌是全球范围内最常见的恶性肿瘤之一。爱泼斯坦-巴尔病毒相关胃癌（Epstein-Barr virus-associated gastric cancer）约占所有胃癌病例的10%。EBV自身可表达多种蛋白质，并调控宿主基因的表达。本研究分别在AGS-EBV细胞中敲低BC200与eIF4A3，并分析其对宿主mRNA表达的影响。整体实验设计：采用Trizol试剂从9份独立培养的AGS-EBV细胞中提取总RNA。