The RNA-seq of HCC specimens and the RIP-seq of HNRNPD in PLC cells and WT (DMSO or Compound C) and circLARP1B-Def (DMSO) PLC/PRF/5 cells

To investigate functional transcripts in metastatic HCC, we performed high-throughput RNA sequencing (RNA-seq) of tumors from 3 metastatic HCC and 3 non-metastatic HCC. And we performed RIP-seq human PLC/PRF/5 cells to investigate the HNRNPD binding transcripts. To investigate function of circLARP1B on AMPK pathway, we performed high-throughput RNA sequencing (RNA-seq) of WT (DMSO or Compound C) and circLARP1B-Def (DMSO) PLC/PRF/5 cells. We performed ribosomal RNA (rRNA) depleted RNA sequencing (RNA-seq) of HCC tissues and PLC cells. We performed ribosomal RNA (rRNA) depleted RNA sequencing (RNA-seq) of PLC/PRF/5 cells.为探究转移性肝细胞癌（hepatocellular carcinoma, HCC）中的功能性转录本，我们对3例转移性肝细胞癌与3例非转移性肝细胞癌的肿瘤组织实施了高通量RNA测序（RNA sequencing, RNA-seq）。为探究结合异质核糖核蛋白D（heterogeneous nuclear ribonucleoprotein D, HNRNPD）的转录本特征，我们对人PLC/PRF/5细胞开展了RNA免疫沉淀测序（RNA Immunoprecipitation Sequencing, RIP-seq）。为明确环状RNA LARP1B（circular RNA LARP1B, circLARP1B）对腺苷酸活化蛋白激酶（AMP-activated protein kinase, AMPK）通路的调控功能，我们分别对经二甲基亚砜（dimethyl sulfoxide, DMSO）或化合物C处理的野生型（wild type, WT）细胞，以及仅经二甲基亚砜处理的circLARP1B缺陷型（circLARP1B-deficient, circLARP1B-Def）PLC/PRF/5细胞开展了高通量RNA测序（RNA-seq）。此外，我们对肝细胞癌组织与PLC细胞进行了核糖体RNA（ribosomal RNA, rRNA）去除型RNA测序（RNA-seq），并对PLC/PRF/5细胞实施了核糖体RNA去除型RNA测序（RNA-seq）。