plasma membrane anchored NAA60 N-terminal acetylation profiling in Arabidopsis thaliana

N-terminal acetylation (NTA) is one of the most abundant protein modifications in eukaryotes and is catalysed in humans by seven N-acetyltransferases. AtNAA60 localizes to the plasma membrane in vivo by an α-helical membrane anchor at its C-terminus. In this study, we investigated the Arabidopsis thaliana N-terminal acetylome in leaf cells of naa60-1 to identify AtNAA60 substrates in vivo using the SILProNAQ approach.

N末端乙酰化（N-terminal acetylation，NTA）是真核生物中丰度最高的蛋白质修饰之一，在人体中由7种N-乙酰转移酶催化。AtNAA60通过其C末端的α螺旋膜锚定序列在体内定位于质膜。本研究以naa60-1突变体的叶细胞为实验材料，对拟南芥（Arabidopsis thaliana）的N末端乙酰化组进行分析，采用SILProNAQ方法在体内鉴定AtNAA60的底物蛋白。