Additional file 1 of Sanguinarine chloride induces ferroptosis by regulating ROS/BACH1/HMOX1 signaling pathway in prostate cancer

Additional file 1: Fig.S1 Effects of compounds on DU145 cells viability.DU145 cells were treated with 10 μM compounds for 48 h. Then, CCK8 assay performed for cell viability assessment. Fig.S2 Effects of compounds on ezalutamide resistance prostate cancer cells viability.22RV1-enz and LNCaP-enz cells were treated with 5 μM compounds for 48 h. Then, CCK8 assay performed for cell viability assessment. Fig. S3 Effect of compoundsand docetaxel on DU145 cells viability.DU145 cells were treated with 5 μM compounds and 2.5 nM docetaxel for 48 h. Then, CCK8 assay performed for cell viability assessment. Fig. S4 S.C triggers ferroptosis in prostate cancer cell. Western blotting analysis of SLC7A11 and GPX4 levels in PC3 a, c and DU145 b, d cells receiving S.C (0, 0.5, 1.0μM) treatment for 48 h. protein levels of HMOX1 measured by western blot in DU145 HMOX1 knockdown cells e. MDA level were detected in PC3 and DU145 cells treated with S.C or erastin for 48h f. SLC7A11 and GPX4 levels were detected in PC3 and DU145 cells treated with S.C or erastin for 48h g.

附加文件1：图S1 化合物对DU145细胞存活率的影响。将DU145细胞以10 μM的化合物处理48小时后，采用CCK8检测法（Cell Counting Kit-8 assay）评估细胞存活率。
图S2 化合物对恩扎卢胺（Enzalutamide）耐药前列腺癌细胞存活率的影响。将22RV1-enz与LNCaP-enz细胞以5 μM的化合物处理48小时，随后通过CCK8检测法评估细胞存活率。
图S3 化合物与多西他赛（Docetaxel）对DU145细胞存活率的影响。将DU145细胞以5 μM化合物及2.5 nM多西他赛处理48小时，再通过CCK8检测法评估细胞存活率。
图S4 S.C可诱导前列腺癌细胞发生铁死亡（Ferroptosis）：对经0、0.5、1.0 μM S.C处理48小时的PC3（a、c）与DU145（b、d）细胞，采用蛋白质印迹法（Western Blotting）检测溶质载体家族7成员11（SLC7A11）与谷胱甘肽过氧化物酶4（GPX4）的蛋白水平；对敲低血红素加氧酶1（HMOX1）的DU145细胞，采用蛋白质印迹法检测其HMOX1的蛋白水平（e）；对经S.C或埃拉斯汀（Erastin）处理48小时的PC3与DU145细胞，检测丙二醛（Malondialdehyde, MDA）水平（f）；对经S.C或埃拉斯汀处理48小时的PC3与DU145细胞，采用蛋白质印迹法检测SLC7A11与GPX4的蛋白水平（g）。