Phage LeviOr01 ssRNA levivirus genome and sequence of Pseudomonas aeruginosa PcyII-10 carrier variants

ssRNA bacteriophages are very abundant but poorly studied, particularly in relation to their effect on bacterial evolution. Leviviridae have the capacity to establish a carrier state in which phages are continuously released, constituting a reservoir and exerting pressure on bacteria. We have isolated from hospital waste water a novel Pseudomonas aeruginosa levivirus, vB_PaeL_PcyII-10_LeviOr01. Its size is 3669 nucleotides and it encodes four putative proteins. Following bacterial infection, a carrier state is established in a fraction of cells conferring superinfection immunity and favoring the emergence of type IV pilus receptor mutants, but also of mutations affecting LPS biosynthesis. The study hosts whole genome sequence from the levivirus, the sequencing reads used to assemble the sequence, and sequencing reads from four P. aeruginosa strain PcyII-10 colonies carrying the virus.

单链RNA噬菌体（ssRNA bacteriophages）分布极为广泛，但相关研究却较为匮乏，尤其是在其对细菌演化的影响层面。勒维噬菌体科（Leviviridae）的噬菌体具备建立携带状态的能力：在此状态下噬菌体可持续释放，形成病毒储存库并对细菌施加选择压力。本研究从医院废水中分离得到一株新型铜绿假单胞菌勒维噬菌体vB_PaeL_PcyII-10_LeviOr01。该噬菌体基因组全长3669个核苷酸，共编码4种推定蛋白。当细菌被该噬菌体感染后，部分宿主细胞会建立携带状态，该状态可赋予宿主对超感染的免疫性，同时促进IV型菌毛受体突变体的产生，亦会催生影响脂多糖（LPS，lipopolysaccharide）生物合成的突变。本数据集包含该勒维噬菌体的全基因组序列、用于组装该基因组序列的测序读段，以及来自4株携带该病毒的铜绿假单胞菌PcyII-10菌株菌落的测序读段。