Influence of temperature and packaging type on quality parameters and antimicrobial properties during Yateí honey storage

Abstract Tetragonisca fiebrigi, is a bee of the subfamily Meliponidae traditionally known as Yateí. Its honey differs from the honey produced by Apis mellifera because it is less viscous, more acidic, has sweetness and particular aromas. It is important to know the behavior of yatei honey in different storage conditions, in order to preserve the characteristics of the honey and ensure the product as harmless. The objective was to determine the influence of temperature, packaging type and storage time of Yateí honey on antimicrobial properties, microbial (Total Mesophilic Aerobic and Mold and Yeast Count) and physicochemical parameters (pH, hydroxymethylfurfural (HMF), acidity, moisture content and diastase), with methodology of national and international standards. The antimicrobial effect was variable against strains of the genus Staphylococcus aureus. The most significant microbiological quality levels were Mold and Yeast counts (> 102 CFU / g). The physicochemical parameters with the most significant values were acidity (to: 42.5 meq acid/kg honey), moisture content (to: 26%) and HMF (to: 3.8mg/kg honey). Storage at refrigeration temperature maintained standard values close to Apis mellifera honey during 180 days; whereas at room temperature it was better to maintain antimicrobial power. No significant difference was found between plastic storage containers vs. glass.

摘要：费布里吉无刺蜂（Tetragonisca fiebrigi）属于无刺蜂亚科（Meliponidae），传统上被称为雅泰伊蜂（Yateí）。其产出的蜂蜜与西方蜜蜂（Apis mellifera）蜂蜜存在显著差异：该蜂蜜粘度更低、酸度更高，兼具甜味与独特香气。为保留蜂蜜固有特性并确保产品安全无害，探究雅泰伊蜂蜂蜜在不同储存条件下的性状变化至关重要。本研究依据国内外标准方法，旨在明确温度、包装类型及储存时长对雅泰伊蜂蜂蜜抗菌性能、微生物指标（总需氧嗜温菌计数、霉菌与酵母菌计数）及理化指标（pH值、羟甲基糠醛（HMF）、酸度、水分含量与淀粉酶活性）的影响。研究发现，其针对金黄色葡萄球菌（Staphylococcus aureus）菌株的抗菌效果存在差异；霉菌与酵母菌计数（＞102 CFU/g）是影响微生物品质的最显著指标。理化指标中变化最显著的为酸度（达42.5 meq酸/kg蜂蜜）、水分含量（达26%）及羟甲基糠醛（HMF，达3.8mg/kg蜂蜜）。在冷藏温度下储存180天，其各项指标可维持在接近西方蜜蜂蜂蜜的标准范围内；而室温储存则更有利于保留蜂蜜的抗菌活性。塑料储存容器与玻璃容器的储存效果无显著差异。